PAPERS
2019
Khoury ME, Winterstein T, Weber W, Stein V, Schlaak HF, Thiel G. Photolithographic Fabrication of Micro Apertures in Dry Film Polymer Sheets for Channel Recordings in Planar Lipid Bilayers. J Membr Biol. 2019 Mar 12. doi: 10.1007/s00232-019-00062-9. https://link.springer.com/article/10.1007%2Fs00232-019-00062-9
Hartel AJW, Shekar S, Ong P, Schroeder I, Thiel G, Shepard KL. High bandwidth approaches in nanopore and ion channel recordings – A tutorial review. Anal Chim Acta. 2019 Jul 11;1061:13-27. doi: 10.1016/j.aca.2019.01.034. Epub 2019 Jan 25. https://www.sciencedirect.com/science/article/pii/S0003267019301035?via%3Dihub#undfig1
2018
Alberio L, Locarno A, Saponaro A, Romano E, Bercier V, Albadri S, Simeoni F, Moleri S, Pelucchi S, Porro A, Marcello E, Barsotti N, Kukovetz K, Boender AJ, Contestabile A, Luo S, Moutal A, Ji Y, Romani G, Beltrame M, Del Bene F, Di Luca M, Khanna R, Colecraft HM, Pasqualetti M, Thiel G, Tonini R, Moroni A. A light-gated potassium channel for sustained neuronal inhibition. Nat Methods. 2018 Nov;15(11):969-976. doi: 10.1038/s41592-018-0186-9. Epub 2018 Oct 30. https://www.nature.com/articles/s41592-018-0186-9
Rauh O, Hansen UP, Scheub DD, Thiel G, Schroeder I. Site-specific ion occupation in the selectivity filter causes voltage-dependent gating in a viral K+ channel. Sci Rep. 2018 Jul 10;8(1):10406. doi: 10.1038/s41598-018-28751-w. https://www.nature.com/articles/s41598-018-28751-w#Sec2
V. Rondelli, E. Del Favero, P. Brocca, G. Fragneto, M. Trapp, L. Mauri, M.G. Ciampa, G. Romani, C.J. Braun, L. Winterstein, I. Schroeder, G. Thiel, A. Moroni, L. Cantu’. Directional K+ channel insertion in a single phospholipid bilayer: Neutron reflectometry and electrophysiology in the joint exploration of a model membrane functional platform. BBA – General Subjects 1862 (2018) 1742–1750. http://dx.doi.org/10.1016/j.bbagen.2018.05.007 https://www.sciencedirect.com/science/article/pii/S0304416518301399?via%3Dihub
Saponaro A, Cantini F, Porro A, Bucchi A, DiFrancesco D, Maione V, Donadoni C, Introini B, Mesirca P, Mangoni ME, Thiel G, Banci L, Santoro B, Moroni A. A synthetic peptide that prevents cAMP regulation in mammalian hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. Elife. 2018 Jun 20;7. pii: e35753. doi: 10.7554/eLife.35753. https://elifesciences.org/articles/35753
Gross C, Saponaro A, Santoro B, Moroni A, Thiel G, Hamacher K. Mechanical transduction of cytoplasmic-to-transmembrane-domain movements in a hyperpolarization-activated cyclic nucleotide-gated cation channel. J Biol Chem. 2018 Aug 17;293(33):12908-12918. doi: 10.1074/jbc.RA118.002139. Epub 2018 Jun 23. http://www.jbc.org/content/293/33/12908
Winterstein LM, Kukovetz K, Rauh O, Turman DL, Braun C, Moroni A, Schroeder I, Thiel G. Reconstitution and functional characterization of ion channels from nanodiscs in lipid bilayers. J Gen Physiol. 2018 Apr 2;150(4):637-646. doi: 10.1085/jgp.201711904. Epub 2018 Feb 27. http://jgp.rupress.org/content/150/4/637.long
Hartel AJW, Ong P, Schroeder I, Giese MH, Shekar S, Clarke OB, Zalk R, Marks AR, Hendrickson WA, Shepard KL. Single-channel recordings of RyR1 at microsecond resolution in CMOS-suspended membranes. Proc Natl Acad Sci U S A. 2018 Feb 20;115(8):E1789-E1798. doi: 10.1073/pnas.1712313115. Epub 2018 Feb 5. https://www.pnas.org/content/115/8/E1789
Timo Greiner, Anna Moroni, James L Van Etten and Gerhard Thiel. Genes for Membrane Transport Proteins: Not So Rare in Viruses. Viruses 2018, 10(9), 456; https://doi.org/10.3390/v10090456 https://www.mdpi.com/1999-4915/10/9/456
2017
Rauh O, Hansen UP, Mach S, Hartel AJW, Shepard KL, Thiel G, Schroeder I. Extended beta distributions open the access to fast gating in bilayer experiments-assigning the voltage-dependent gating to the selectivity filter. FEBS Lett. 2017 Dec;591(23):3850-3860. doi: 10.1002/1873-3468.12898. http://onlinelibrary.wiley.com/doi/10.1002/1873-3468.12898/abstract
Saponaro A, Porro A, Chaves-Sanjuan A, Nardini M, Rauh O, Thiel G, Moroni A. Fusicoccin Activates KAT1 Channels by Stabilizing Their Interaction with 14-3-3 Proteins. Plant Cell. 2017 Oct;29(10):2570-2580. doi: 10.1105/tpc.17.00375. http://www.plantcell.org/content/29/10/2570.long
Rauh O, Urban M, Henkes LM, Winterstein T, Greiner T, Van Etten JL, Moroni A, Kast SM, Thiel G, Schroeder I. Identification of Intrahelical Bifurcated H-Bonds as a New Type of Gate in K+ Channels. J Am Chem Soc. 2017 Jun 7;139(22):7494-7503. doi: 10.1021/jacs.7b01158. https://pubs.acs.org/doi/10.1021/jacs.7b01158
Cosentino C, Alberio L, Thiel G, Moroni A. Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels. Methods Mol Biol. 2017;1596:271-285. doi: 10.1007/978-1-4939-6940-1_17. https://link.springer.com/protocol/10.1007%2F978-1-4939-6940-1_17
Weißgraeber S, Saponaro A, Thiel G, Hamacher K. A reduced mechanical model for cAMP-modulated gating in HCN channels. Sci Rep. 2017 Jan 11;7:40168. doi: 10.1038/srep40168. https://www.nature.com/articles/srep40168